齊一生物大鼠D-乳酸檢測(cè)試劑盒價(jià)格:全國(guó):或咨詢?cè)诰€客服,或者以郵件形式發(fā)到我司741653262,齊一生物科技(上海)有限公司提供大量品牌進(jìn)口原裝,分裝,以及穩(wěn)定性強(qiáng)的國(guó)產(chǎn)ELISA試劑盒,性價(jià)比*
齊一生物大鼠D-乳酸檢測(cè)試劑盒標(biāo)本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存 過(guò)程 中 如有沉淀形成,應(yīng)再次離心。
2.血漿:應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成,應(yīng)再次離心。
3.尿液:用無(wú)菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成,應(yīng)再次離心。胸腹水、腦脊液參照此實(shí)行。
4.細(xì)胞培養(yǎng)上清:檢測(cè)分泌性的成份時(shí),用無(wú)菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/ 分)。仔細(xì)收集上清。 檢測(cè)細(xì)胞內(nèi)的成份時(shí),用PBS(PH7.2-7.4)稀釋細(xì)胞懸液,細(xì)胞濃度達(dá)到100萬(wàn)/ml左右。通過(guò)反復(fù)凍融,以 使細(xì)胞破壞并 放出細(xì)胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過(guò)程中如有沉淀形成,應(yīng)再次離心。
5.組織標(biāo)本:切割標(biāo)本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆?。?biāo)本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。分裝后一份待檢測(cè),其余冷凍備用。
標(biāo)本要求:
1.標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過(guò)氧化物酶的(HRP)活性。
試劑盒組成:
48孔配制:
1. 說(shuō)明書(shū): 1份
2. 封板膜: 2片(48)
3. 密封袋: 1個(gè)
4 酶標(biāo)包被板: 1×48 (2-8℃保存)
5. 標(biāo)準(zhǔn)品:0.5ml×1瓶(2-8℃保存)
6. 標(biāo)準(zhǔn)品稀釋液: 1.5ml×1瓶(2-8℃保存)
7. 酶標(biāo)試劑: 3 ml×1瓶(2-8℃保存)
8. 樣品稀釋液: 3 ml×1瓶(2-8℃保存)
9. 顯色劑A液: 3 ml×1瓶(2-8℃保存)
10 .顯色劑B液: 3 ml×1瓶(2-8℃保存)
11 .終止液:3ml×1瓶(2-8℃保存)
12. 濃縮洗滌液:(20ml×20倍)×1瓶(2-8℃保存)
9020317 PM kit, C18 & QIAxtractor (HEPA), CX1
9017245 Microplate Station 8000, seesaw
9020226 Belt, X-axis assembly, C4x
9020211 Switch, assembly, lid, Nov08-Jun09, C1x
9020210 Foot assembly, QGX
9020154 USB Connector&Cable; RGQ
9020091 Hall Sensor Board, TLyser 2
9239514 EX SD Controller
9239512 EX XY, Platter Interconnect
9239509 EX XY, Controller Board XYP
9236529 Tubing Guide, Probe Holder, BR8 RD
9236512 #BR 9604, PM Service The Overhaul covers all travel and labor costs plus all necessary parts to perform a Preventative Maintenance,. The instrument will also be thoroughly checked and inspected to determine if any additional parts are required to get the instrument running to its optimal performance. There will be an additional charge for any such parts if any are needed.
9232778 Card, x-main, PCON, 2
9239314 Reservoir, OLF, with sensor, RapidPlate
9236449 Travel Charge, zone D
9236434 Sensor, rotary home, TWI
9239174 Cooling Block , 24-tube, 1.5 ml, tall
9232634 Bottle, 250-ml, blue fitting
9017157 BR MDx CTM 48 PCR Setup, PRO
9232475 Cover, SIS, BR9604, V1.01
9232334 Cable, magnet valve, DISU, BR8000
9232331 Cable, power, dilutor 5-8, BR8000
9238779 Agreement, MDx, 2PM, 1yr An agreement for preventive maintenance provides coverage for travel, labor, and parts, plus the cost of consumables required for two maintenance visits during 1 year. Work carried out during the preventive maintenance visit is fully documented in accordance with GMP/GLP directives.
9238773 Project Pack Includes 12 working days (on-site or off-site work) by a QIAGEN Instrumentation Service Specialist, valid for 3 years. These days can be used for writing and/or optimization of protocols or for training. It includes all travel and labor charges. Parts, consumable chemistries, and accessories are not included. Custom protocols are the sole responsibility of the customer and neither warrantied nor guaranteed.
9238911 Power supply 40W, BS96
9238743 Connector, tubing, 1/8 in to 6 mm , V2
9238711 Service manual Zent1/2, english
9238891 Fan assembly, BS96
9238902 Micro switch-cable assembly 1, BS96
9238701 Mains switch
9238692 Relay 1U 24 V
9232098 Front blind for size 90
9238678 Socket key, release of lid locks Square wrench "Emergency lid release" for SIGMA 4-15C ; SIGMA part # 80054
9231031 Cable, card-X to pressure sensor, 950 mm
9020077 Screw M8x20, Ratchet Holder, TLyser
9020066 Support Plate for Brake, TLyser
9022499 EZ1 Adv.DNA Invest.Large Scale Bone Card
9239153 Power entry module, (TW9940N0663), TWI
9239027 RCS O-Rings, RCS1
9232694 Pump head, MCP/CA4, "click'n'go"
9232632 Bottle, 250-ml, black fitting
9232613 Holder, external bottle, rectangular
9232589 Cooling block, 500ml
9238949 Track sensor, BS15
9238796 Rectangular bucket
9232548 Power Cord, Australia
9238944 Power supply 300 W, BS15
9238928 Tooth belt wheel, BS15
9238768 Swing out rotor for 4 buckets Sigma Rotor # 11140
9238751 Glases, Laser protection, 530-650nm
9238709 Key with ring
9238901 Track sensor, BS96
9238893 Magnet, BS15
9231891 Lid, 1-8 connector, BR8000
9238684 Eprom and controller 4-15, Zent 3C
9231633 Connector, top, dispenser head, BR8000
9230932 SlidingSheet, back, 12-Samtrack
9020079 Loctite 241,10 ml, TLyser
9020051 V-Belt, TLyser
9020050 Eccentric Shaft, TLyser
9015009 DT-Adapter, 1100μl, BR8, v4.03
9014851 Dilutor unit, BR8 RD
9014603 PCB, South-Edge, BR8, RD
9022321 RS-232 Cable (2), careHPV
9022210 Alignment Tool, Reagent Probe, DCU
9022120 Tool, Rotor Mounted LED lightsource, RGx
9022098 Alignment Tool Kit, DCU
9021978 Pneumatic Manifold, QXA
9021693 Gas strut, Pyro Q24
9021636 Waste box, blue, w yellow lid, Qsym
9238490 Motor, vertical, replacement kit, RP
9238221 Fitting Valve (5)
9240673 Kerk Nut, Capper Up/Down, AP196/98
9240667 Nut, Flangeless,1/4-28, Green, AP
9240664 Nut, Flangeless,1/4-28, Red, AP
9240596 MAC Valve, Manifold, Pressure, AP98
9013864 QIAsoft 4.x, BR3000 Cust. Edition
9021192 Cover, transparent, BS96
9021113 Skin Panel, Upper Right Side, DCU
9021109 Skin Panel, Upper Center Front, DCU
9021104 Skin Panel, Lower Right Side, DCU
TIN2 Polyclonal Antibody Human WB IHC ELISA
Tip5 Polyclonal Antibody Human WB ELISA
TIP60 Polyclonal Antibody Human, Mouse IHC IF ELISA
TIP60 Polyclonal Antibody Human, Mouse, Rat IHC ELISA
TIP60 Polyclonal Antibody Human, Mouse, Rat WB ELISA
TIRAP Polyclonal Antibody Human, Mouse IHC ELISA
TIS11B Polyclonal Antibody Human, Mouse, Rat WB IHC ELISA
TIS11D Polyclonal Antibody Human, Mouse WB IHC ELISA
TLE2 Polyclonal Antibody Human WB IHC ELISA
TLE4 Polyclonal Antibody Human, Mouse, Rat WB IHC ELISA
TLK1 Polyclonal Antibody Human WB ELISA
TLK1 Polyclonal Antibody Human, Mouse IF ELISA
TLK2 Polyclonal Antibody Human, Mouse WB ELISA
TM4SF1 Polyclonal Antibody Human, Mouse IHC ELISA
TMC8 Polyclonal Antibody Human, Mouse, Rat WB IHC ELISA
TMEM101 Polyclonal Antibody Human, Mouse WB ELISA
TMEM145 Polyclonal Antibody Human, Mouse WB IHC IF ELISA
TMEM16A Polyclonal Antibody Human IF ELISA
TMEM185A Polyclonal Antibody Human, Mouse WB IHC IF ELISA
TMEM37 Polyclonal Antibody Human IHC ELISA
TMPRSS3 Polyclonal Antibody Human, Mouse WB IHC IF ELISA
TNAP Polyclonal Antibody Human, Mouse, Rat WB ELISA
TNF-IP 1 Polyclonal Antibody Human, Mouse, Rat WB IHC ELISA
TNF-IP 8 Polyclonal Antibody Human, Mouse WB IHC IF ELISA
TNF-IP 8L2 Polyclonal Antibody Human, Mouse, Rat WB ELISA
TNF-R1 Polyclonal Antibody Human, Mouse, Rat IHC ELISA
TNF-R2 Polyclonal Antibody Human, Mouse, Rat WB IHC IF ELISA
TNF-α Polyclonal Antibody Human, Mouse, Rat IHC IF ELISA