人Ⅰ類主要組織兼容性復合體G檢測試劑盒定量檢測,具有靈敏性高,特異性強,重復性好,性價比高,線性好;操作簡單,精確度高;售前售后完善,隨時來實驗室現(xiàn)場觀看,常見ELISA試劑盒系列:人,大鼠,小鼠:TNF-α、IL-1β、IL-7、IL-32、IL-21、IL-27、IL-23、IFN-γ、TGF-α、TGF-β1等。
英文簡稱:Human Major Histocompatibility Complex Class I G (MHCG) ELISA Kit
人Ⅰ類主要組織兼容性復合體G檢測試劑盒注意事項以下四點:
1,封閉:以下包以后是無關的蛋白質(zhì)溶液濃度高,涂層技術。隨函附上使許多不相關的蛋白質(zhì)充填這些空隙,和攪擾物質(zhì)的免疫排擠和吸附進程。ELISA試劑盒常用的密封:0。05% - 0。5%牛血清白蛋白;10%或1%明膠牛血清;脫脂奶粉,報價相對低廉,可用于高濃度(5%~10%);和一些稀有的運用各種動物血清(首要是為了消除類似的蛋白和酪蛋白等攪擾)。但究竟挑選啥,依據(jù)試驗的詳細實習。
2,洗板:能夠說,在中操作,清洗是在首要的關鍵技術。因為聚苯乙烯等塑料對蛋白質(zhì)的吸附是普遍存在的,為了完成自在酶與客觀相的符號的別離,在板料孔中殘留游離和吸附的非特異性攪擾物質(zhì)的去掉,洗刷,并應將攪擾物質(zhì)洗刷下來的非特異性吸附。所以在洗衣板會有必定的誤差,非常人的要素(當然也有洗衣機除條件),是不完整的或弦清孔,體系的影響是如此靈敏,但不小。
3,加抗體(抗標本和兩個):留意的槍頭,槍頭。樣品用稀釋稀釋,也能夠用稀釋的閉解。假如要增加兩個電阻,但也要留意兩個反作業(yè)濃度的廢物,太高,太低的光的色彩。
4,色彩:色彩體系有許多,咱們開端做,挑選恰當?shù)纳鼠w系。ELISA試劑盒留意色彩體系酶底物物加硫柳泵節(jié)省,組合物加*。7,每次都盡可能的陰和陽空三操控,如剖析疑問的呈現(xiàn)。YB-PDI-Hu 人蛋白二硫鍵異構酶(PDI) ELISA Kit Human Protein Disulfide Isomerase (PDI) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.127ng/mL sandwich 96T/48T 926.3/648.5 12 months
YB-PDK1-Ra 大鼠丙酮酸脫氫酶激酶同工酶1(PDK1) ELISA Kit Rat Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.057ng/mL sandwich 96T/48T 1045.1/731.6 12 months
YB-PDLIM1-Hu 人PDZ和LIM域蛋白1(PDLIM1) ELISA Kit Human PDZ And LIM Domain Protein 1 (PDLIM1) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.061ng/mL sandwich 96T/48T 1057.5/740.3 12 months
YB-PDP-Hu 人丙酮酸脫氫酶磷酸酶(PDP) ELISA Kit Human Pyruvate Dehydrogenase Phosphatase (PDP) ELISA Kit Homo sapiens human 0.312-20ng/mL 0.099ng/mL sandwich 96T/48T 882.5/617.7 12 months
YB-PDPK1-Mu 小鼠磷酸肌醇依賴性蛋白激酶1(PDPK1) ELISA Kit Mouse Phosphoinositide Dependent Protein Kinase 1 (PDPK1) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.055ng/mL sandwich 96T/48T 995/696.5 12 months
YB-PDP-Mu 小鼠丙酮酸脫氫酶磷酸酶(PDP) ELISA Kit Mouse Pyruvate Dehydrogenase Phosphatase (PDP) ELISA Kit Mus musculus mouse 3.125-200U/L 1.3U/L sandwich 96T/48T 905/633.5 12 months
YB-PDPN-b 牛平足蛋白(PDPN) ELISA Kit Bovine Podoplanin (PDPN) ELISA Kit Bos taurus; Bovine Cattle 0.312-20ng/mL 0.078ng/mL sandwich 96T/48T 1145/801.5 12 months
YB-PDPN-Hu 人平足蛋白(PDPN) ELISA Kit Human Podoplanin (PDPN) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.056ng/mL sandwich 96T/48T 970.1/679.1 12 months
YB-PDPN-Mu 小鼠平足蛋白(PDPN) ELISA Kit Mouse Podoplanin (PDPN) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.055ng/mL sandwich 96T/48T 995/696.5 12 months
YB-PDPN-Ra 大鼠平足蛋白(PDPN) ELISA Kit Rat Podoplanin (PDPN) ELISA Kit Rattus norvegicus Rat 0.156-10ng/mL 0.053ng/mL sandwich 96T/48T 1045.1/731.6 12 months
YB-PEBP1-Hu 人磷脂酰乙醇胺結合蛋白1(PEBP1) ELISA Kit Human Phosphatidylethanolamine Binding Protein 1 (PEBP1) ELISA Kit Homo sapiens human 0.156-10ng/mL 0.058ng/mL sandwich 96T/48T 970.1/679.1 12 months
YB-PEBP1-Mu 小鼠磷脂酰乙醇胺結合蛋白1(PEBP1) ELISA Kit Mouse Phosphatidylethanolamine Binding Protein 1 (PEBP1) ELISA Kit Mus musculus mouse 0.156-10ng/mL 0.055ng/mL sandwich 96T/48T 995/696.5 12 months
YB-PECAM1-Hu 人血小板內(nèi)皮細胞粘附分子1(PECAM1) ELISA Kit