小鼠致癌基因相關(guān)蛋白KCELISA試劑盒FOR RESEARCH USE ONLY
Drug Names
Generic Name：小鼠致癌基因相關(guān)蛋白KCELISA試劑盒
This kit can be used for determination of serum, plasma and liquid samples Organization Content.
The experimental principle:
The product levels were measured in samples of the kit by double antibody sandwichmethod. The product with the purified antibody coated microtiter plate, made of solid phase antibody, to package is the product antigen monoclonal antibodies are then added to the micropores, the product and then with HRP labeled antibody binding, the formation of antibody - antigen - antibody complex enzyme label, after thorough washing with TMB chromogenic substrate. TMB in the HRP enzyme catalytic conversion into the blue, and in the action of acid into the final yellow. This product is positively related to the depth of color and in the samples. Instrument measured absorbance in the 450nm wavelength with ELISA (OD), the product concentration in the samples was calculated by standard curve.
Materials provided with the kit
Materials provided with the kit    48determinations    96 determinations    Storage
User manual    1    1    
Closure plate membrane    2    2    
Sealed bags    1    1    
Microelisa stripplate    1    1    2-8℃
Standard：360ng/L    0.5ml×1 bottle    0.5ml×1 bottle    2-8℃
Standard diluent    1.5ml×1 bottle    1.5ml×1 bottle    2-8℃
HRP-Conjugate reagent    3ml×1 bottle    6ml×1 bottle    2-8℃
Sample diluent    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution A    3ml×1 bottle    6ml×1 bottle    2-8℃
Chromogen Solution B    3ml×1 bottle    6ml×1 bottle    2-8℃
Stop Solution    3ml×1 bottle    6ml×1 bottle    2-8℃
wash  solution    （20ml×20 fold）
×1bottle    （20ml×30 fold）
×1bottle    2-8℃
Specimen requirements
1.serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
2.plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
3.Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.
4.cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.
5.Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.
6.extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
7.Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
Assay procedure
1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separay. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 240ng/L,160ng/L ,80ng/L,40ng/L, 20ng/L)
2.add sample：Set blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold (or 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except  blank well. 
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
Important notes
1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.
2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.
3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 mins, if the number of sample is much , recommend to use Volley .
4.if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.（×n×5）.
5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.
6.The substrate evade the light preservation.
7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.
8.All samples, washing buffer and each kind of reject should according to infective material process.
9.Do not mix reagents with those from other lots.
Calculate：
Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.
Storage and validity
1．Storage：  2-8℃.
2．validity： six months.

其它產(chǎn)品159155    QIAsafe DNA Blood Rehydration Set (100)
158845    Gentra Puregene Buccal Cell Kit (100)
158667    Gentra Puregene Tissue Kit (4 g)
34610    RGS·His Antibody (100 µg)    100 ug mouse anti-RGS(H)4 (lyophilized, with BSA, for 1000 ml working solution)
33903    pQE-TriSystem Vector DNA    25 ug pQE-TriSystem Vector DNA
32723    EasyXpress Linear Template Kit Plus (20)
32502    EasyXpress Protein Synthesis Kit (20)    For 20 x 50 ul reactions: E. coli extract, reaction buffer, RNase-free water, and positive-control DNA
30760    Ni-NTA Superflow Cartridges (1x5 ml)
30410    Ni-NTA Superflow (25 ml)    25 ml nickel-charged resin (max. pressure: 140 psi)
9021097    Sensor, Proximity SME-8M, DCU
9021218    # LED, 4 µA (12), QX
9021351    Piston pin, connecting rod, TLyser lT
28206    MinElute Reaction Cleanup Kit (250)    250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
9243307    HC2 Systems Training – On site    HC2 On site, 3 day course trains users (max. 1 participant) on the HC2 system (manual or automated) and includes trainer travel, time and materials.
101.572-12U    #HLA-B*5701 excl. Taq (12)
6000-3002    #Washer Alignment Block, PTW1
9011231    Tischplatte Plato 3101 SI Immucor (9194)
9011207    High speed shaker, autom. plate, BR8000
9011002    Base,rotor motor,buff.track,BR8000 v1.00
1013366    Ni-NTA HisSorb 384 Plates, white (100)
150345    REPLI-g Single Cell Kit (96)
150045    REPLI-g Midi Kit (100)    DNA Polymerase, Buffers, and Reagents for 100 x 50 µl whole genome amplification reactions (typical yield 40 µg per reaction)
150033    REPLI-g UltraFast Mini Kit (25)    DNA Polymerase, Buffers, and Reagents for 25 x 20 μl ultrafast whole genome amplification reactions
9235275    Filter, condensate bottle, auto vac, BR8
9235120    Fuse T5A, v1.00
9235052    Splashing Cover , Wash Station RCS
9234888    Vacuum manifold, 6s seal
9234859    Tubing coupling piece waste station tub.
9238665    Pneumatic spring, 125 Nm
9238644    Varistor S20 K250
9238624    Eprom and controller 4K15, Zent3
9242065    QIAxcel Rental
9241892    QIArent&go - Rent out fee QIAgility
9241829    Pyro Q96 MD, Premium Agreement    "Repair service delivery: onsite
Service response time (repair): 24 hours/1 business day
Periodic inspection/maintenance: yes, 1/agreement year
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts: yes, included
Cost coverage for labor: yes, included
Cost coverage for travel: yes, included
Replacement system (Loaner) provision: not included
Transportation cost coverage: not applicable"
9241163    Board, motor control, X, QX
9241128    Krytox RFE Lubricant
9241123    Shim Stock
9240984    PCB, p&c, cooling device, Qsym
9240983    Cooling device, eluate drawer, Qsym
9240952    Handler, Qsym
9240950    PCB, xyz, portal, Qsym
9240836    QIAcube, Full Agreement, no PM    "Repair service delivery: onsite
Service response time (repair): 48 hours/2 business days
Periodic inspection/maintenance: not included
Inspection/maintenance delivery: not applicable
Cost coverage for repair parts: yes, included
Cost coverage for labor: yes, included
Cost coverage for travel: yes, included
Replacement system (Loaner) or PC repair provision: not included
Transportation cost coverage: not applicable"
9240684    Pogo Stick Kit, AP196/98
9240802    Performance, IQ/OQ, EZ1
9240782    Update, Weigh Cell, AP196
9240779    500 ml Reagent #6 Update, AP98
9240770    Assy, Capper Shuttle, AP96
9240767    8 OUT Relay, F2-08TRS, AP96
9240765    32 IN Module, D2-32ND3, AP96
9241778    RGQ, Inspection Service    "The Annual Inspection Service, Rotorgene-Q is a Regional Repair Center equipment service, performed by a QIAGEN service specialist. 
All required testing material and certified equipment is provided by the service specialist. 
During the half day-long procedure, all application-critical modules of the Rotorgene are inspected and tested and an OTV check is conducted. Performed tests and test results are documented in a GMP/GLP-compliant Report."
9241730    BR Universal ApplicationPack Veterinary
9241394    C-Nut set, EZ1a
9240749    Belt, Shaker, AP
9243644    Guided Valid Support, QuantiPlex/OPHyres    The QIAGEN Quantiplex/Quantiplex HYres Guided Validation Support service includes all travel and labor charges for a site visit up to 2 days by a QIAGEN Specialist.  A QIAGEN specialist will provide an introduction to the Quantiplex/Quantiplex HYres chemistry utilized in the process, review the Handbook and User Manual, as well as hands-on operation to match the lab’s forensic workflow. The Guided Validation service includes experimental design consulation, internal validation documentation support, and QIAGEN sponsored data templates.  The QIAGEN Specialist will also aide the customer in running some samples to initiate the validation process.  Enough consumable chemistry and accessories (QIAGEN or other vendors) should be purchased by the customer to cover the validation experimental design and are not included in the cost of validation support.
9243642    Pyro Q24, Repair Fee    "Repair service delivery send to Regional Repair Center
Service response time (repair) 7-10 days turnaround time
Periodic inspection/maintenance: 0
Cost coverage for Repair Parts : yes, full coverage
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage"
9243540    LiquiChip, PM Service    "Service response time (repair): not applicable
Periodic inspection/maintenance: yes
Inspection/maintenance delivery: onsite
Cost coverage for Repair Parts : not included
Cost coverage for labor: yes, full coverage
Cost coverage for travel: yes, full coverage
Replacement system (Loaner) provision: not applicable
Transportation cost coverage: yes, full coverage"
9243114    Flow Control Valve, Weigh Cell, AP
9242862    QIArent&go - Rent out fee QSSP Cabinet
9242676    QS RGQ, Installation    The Installation, QIAsymphony RGQ facilitates the quick implementation of the QIAsymphony RGQ into the lab routine by minimizing the instrument start-up phase. It covers the hardware and software installation of QIAsymphony RGQ instruments and provides the user with an introductory training. The installation includes or labor and travel costs. The performance of the installation process is documented on a GMP/GLP-compliant Field Service document.
5000-1324    Motor 230V, SKR1
206045    Type-it Fast SNP Probe PCR Kit (800)    "Features
? Outstanding separation for reliably high call rates
? For difficult SNP loci and for low amounts of template
? Validated using TaqMan SNP Genotyping Assays 
? Up to 40% time savings due to fast-cycling procedure
? Convenient master mix format and optimized protocol"
9001911    PyroMark Q24 MDx, Priority    PyroMark Q24 MDx, Priority includes the Pyromark Q24 MDx system, installation and extended warranty. Installation covers hardware and software installation of system and provides user with on site introductory training. Priority package extends warranty period of the system to 2 years with shipment to Repair Center Service and response turnaround time (repair) 7-10 days. One preventive maintenance visit is included for each year of the warranty; 2 preventive maintenance visits in total. Replacement system (Loaner) provision included (provision time 2-3 days) with transportation cost coverage. The service package includes all parts, labor and travel costs with installation performance and service visits documented on a GMP/GLP-compliant Field Service documents. (In the event of shipping logistic limitation, QIAGEN reserves the option to repair the instrument on-site. Response time 48 Hrs)
965842    QIAamp 96 DNA Swab BioRobot Kit (12)    For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Proteinase K, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps
133186    NeXtalStock Citric acid pH 4.0 (200)
133185    NeXtalStock Citric acid pH 3.8 (200)
9018554    Cover, front, left, EZ1a
9018529    Communicator, Software CD, EZ1a
51206    FlexiGene DNA Kit (250)    For purification of DNA from 250 ml whole blood: Buffers, QIAGEN Protease
51106    QIAamp DNA Blood Mini Kit (250)    For 250 DNA minipreps: 250 QIAamp Mini Spin Columns, QIAGEN Protease, Reagents, Buffers, Collection Tubes (2 ml)
50214    QIAamp UCP Pathogen Mini Kit (50),G
133102    NeXtalStock Potassium thiocyanate (200)
9015517    Thermocouple
9015469    Motor, M
9001879    QIAxcel, PrioPLUS    "QIAxcel Advanced Instrument 
Installation of QIAxcel Advanced & Introductory training 
Warranty extension to 3 yrs
Repair Center service, response 7-10
Loaner available in 2 days*
Preventive Maintenance (onsite or RRC,  1 PM per year)
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service
All cost covered (travel, labor, parts)"
9001876    EZ1 Advanced XL, PrioPLUS    "EZ1 Advanced XL Instrument 
Installation of EZ1 instrument & Introductory training 
Warranty extension to 3 yrs
On site service, 48 hr response
Preventive Maintenance (1 onsite PM per year)
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service
All cost covered (travel, labor, parts)"
9001870    Rotor-Gene Q 6plex (PC) PrioPLUS    "Rotor-Gene Instrument 
Installation of Rotor-Gene & Introductory training 
Warranty extension to 3 yrs
Repair Center service, response 7-10
Loaner available in 2 days*
Annual Inspections (1 onsite PM per yearr)
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service
All cost covered (travel, labor, parts)"
9012471    Side Panel Assembly, Arm, RCS
958236    MagAttract RNA Cell Mini M48 Kit (192)
9018455    Linear guide, Z axis, QX
9018453    Clip, blue, for tubing (20), QX
133166    NeXtalStock CAPS pH 11.6 (200)
9018430    Key, replacement, grey, QX FA
1068797    Buffer DLB (13mg lyophylized), G
1067930    Buffer ALO (10 ml)
133089    NeXtalStock PEG 6,000 (200)
133075    NeXtalStock PEG 1,500 (200)
38051    LCH Cartridge (1ml)
9015381    Unit, heat/cool, right, M96
204652    QuantiFast Multiplex PCR Kit (80)
204556    QuantiFast ProbeRT-PCR+ROXvial Kit(2000)
805943    REPLI-g Service (100 µg)
290085    mericon Quant. Legionella spp Kit (96)
290043    mericon Campylobacter triple Kit (24)
204356    QuantiFast Probe PCR+ROXVial Kit (2000)
204343    QuantiTect Probe PCR Kit (200)    For 200 x 50 ul reactions: 3 x 1.7 ml QuantiTect Probe PCR Master Mix, 2 x 2.0 ml RNase-free water
133290    NeXtalStock TRIS-HCl pH 8.8 (200)
1012829    Effectene Transfection Reagent (100ml)    Effectene ONLY!!!
995904    Reagent Container, large, M48 (50)
337840    qBiomarker SYBR ROX FAST Mastermix (2)
9018910    Rotor-Disc 72 Loading Block
9018901    Loading Block 72 x 0.1 mL Tubes
9018706    EZ1 Adv. XL Test Card
9018566    Cable reels, spring-driven, EZ1a
9013003    Linear guide set, V-handler
673013    ipsogen JAK2 MutaScreen Kit (24)
672313    ipsogen PML-RARA bcr3 Kit (24)