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MIF Human His C/重組人巨噬細(xì)胞移動(dòng)抑制因子，C -端His Tag 產(chǎn)品編號(hào)：CYT-521

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MIF Human His C/重組人巨噬細(xì)胞移動(dòng)抑制因子，C -端His Tag 產(chǎn)品編號(hào)：CYT-521
產(chǎn)品規(guī)格：5μg/25μg/1mg

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MIF Human His C/重組人巨噬細(xì)胞移動(dòng)抑制因子，C -端His Tag

產(chǎn)品編號(hào)：CYT-521
產(chǎn)品規(guī)格：5μg/25μg/1mg
 

Synonyms Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF.
Introduction The cytokine Macrophage migration inhibitory factor （MIF） has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
Description MIF human Recombinant, fused to His-tag at C-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.
Macrophage Inducing Factor Human Recombinant is a single, non-glycosylated, polypeptide chaincontaining 123 amino acidsand having a molecular mass of 13.5 kDa.
Source Escherichia Coli.
Physical Appearance Sterile Filtered lyophilized powder.
Formulation Human MIF was lyophilized from a 1mg/ml solution containing PBS pH-7.4.
Solubility It is recommended to reconstitute the lyophilized MIF in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized MIF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MIF should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE.
Biological Activity Measured by its ability to bind rhCD74 in a functional ELISA.
Amino Acid Sequence MPMFIVNTNVPRASVPDGFLSELTLAQATGKPPQYIAVHVVPDQLMAFGGS
SEPALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVG
WNNSTFALEHHHHHH.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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