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重組小鼠白介素2/IL 2 Mouse 產(chǎn)品編號(hào)：CYT-370

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重組小鼠白介素2/IL 2 Mouse產(chǎn)品編號(hào)：CYT-370
產(chǎn)品規(guī)格：5μg/20μg/1mg

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重組小鼠白介素2/IL 2 Mouse

產(chǎn)品編號(hào)：CYT-370
產(chǎn)品規(guī)格：5μg/20μg/1mg

Catalogue Number	CYT-370
Synonyms	Interleukin-2, T-cell growth factor （TCGF）, Aldesleukin, Lymphokine, IL-2.
Introduction	IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 （IL4） and interleukin 7 （IL7）. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
Description	Interleukin-2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 148 amino acids and having a molecular mass of 17231 Dalton. The IL-2 is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation	The protein （1mg/ml） was lyophilized extensive dialysis against 10mM sodium citrate buffer to a pH=4.
Solubility	It is recommended to reconstitute the lyophilized Mouse IL-2 in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Purity	Greater than 98.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE.
Amino acid sequence	PTSSSTSSST AEAHLEQL LMDLQELLSR MENYRNLKLP RMLTFKFYLP KQAKDLQ CLEDELGPLR HVLDLTQSKS FQLEDAENFI SNIRVTVVKL KGSDNTFECQ FDDESATVVD FLRRWIAFCQ SIISTSPQ.
Biological Activity	The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is 0.18 ng/ml corresponding to a Specific Activity of 55,000,000IU/mg.
Protein content	Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.482 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.