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上海經(jīng)科化學(xué)科技有限公司
上海經(jīng)科化學(xué)科技有限公司
| 參 考 價 | 面議 |
產(chǎn)品型號SD1259
品 牌
廠商性質(zhì)經(jīng)銷商
所 在 地上海市
聯(lián)系方式：李小姐查看聯(lián)系方式
更新時間：2017-07-21 14:49:44瀏覽次數(shù)：504次
聯(lián)系我時，請告知來自 環(huán)保在線暫無信息 |
pRNAT-U6.2/Lenti siRNA expression vector is compatible with the Invitrogen ViraPowerTM Lentiviral_Expression System. It is designed for siRNA expression in both dividing and nondividing cells.
| Description: pRNAT-U6.2/Lenti siRNA expression vector is compatible with the Invitrogen ViraPowerTM Lentiviral_Expression System. It is designed for siRNA expression in both dividing and nondividing cells. The siRNA insert can be conveniently cloned between the BamH I and Xho I sites under the control of the U6 promoter. The vector contains a neomycin resistance gene for establishing stable cell lines and a coral GFP gene for tracking transfection efficiency. Both of these are driven by CM and SV40 promoters, respectively. The vector uses HIV 3'LTR for viral transcription and a Rous sarcoma enhancer/promoter joined to HIV 5'LTR （PRSV/5'LTR） for packaging. |
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| Download Protocol:tm0175 |
| Forward Sequencing Primer: DA0011:pRNA-U6 Forward （TACGATACAAGGCTGTTAGAGAG） |
| Reverse Sequencing Primer: DA0012:pRNA Reverse （TAGAAGGCACAGTCGAGG） |
| Detailed Map: |
| 5' LTR:1-410 |
| 3' LTR:4906-5140 |
| ColE1 ori:7220-8106 |
| Polylinker:2200-2275 |
| U6 Promoter:1873-2199 |
| CMV Promoter:2320-2907 |
| cGFP:2924-3643 |
| SV40:3651-3996 |
| Neomycin:4037-4831 |
| Ampicillin:6300-7160 |
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