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TUNEL Apoptosis Detection Kit （For Paraffin-embedd

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The TUNEL Apoptosis Detection Kit for Paraffin-embedded Tissue Sections (FITC-labeled POD) (Cat. No. L00300) is one of GenScript's newly introduced products.

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Description

The TUNEL Apoptosis Detection Kit for Paraffin-embedded Tissue Sections （FITC-labeled POD） （Cat. No. L00300） is one of GenScript's newly introduced products. The kit can detect fragmented DNA in the nucleus during apoptosis. In this modified TUNEL assay kit, fluorescein-labeled nucleotides bind with the DNA 3'-OH ends using natural or recombinant terminal deoxynucleotidyl transferase （TdT or rTdT）. The fluorescence could be observed by fluorescence microscope. And then the compound of anti-fluorescein antibody and HRP is bound to these fluorescein labeled nucleotides, which are detected using the peroxidase substrate, hydrogen peroxide, and 3,3’-diaminobenzidine （DAB）, a stable chromogen. Using this procedure, apoptotic nuclei are stained dark brown.

Key features

Simplified Procedure: The kit contains ready-to-use reagents, including proteinase K and DAB.
Enhanced Sensitivity: This kit can assay the cells during the early stages of apoptosis.
Enhanced Specificity: The kit can stain apoptotic cells.
Streamlined Process: The entire procedure takes about three hours.
Increased Convenience: The results can be observed by fluorescence microscope and light microscope.