★研盟生物 ★合作: ★客服
相關(guān)標記抗體:HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體
上海研盟生物科技有限公司Anti-Morphine抗體*,主要應用于WB、IHC、IF、ELISA、流式細胞術(shù)等實驗中。說明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/span>
英文名稱:Anti-Morphine antibody
中文名稱:*抗體
*抗體保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
*抗體,Anti-Morphine抗體免疫應答過程:
1.抗原進入機體;
2.抗原刺激 B淋巴細胞,使之分化增殖成為抗體分泌漿細胞和記憶細胞(初次免疫應答);
3.抗體主要由漿細胞分泌 ;
4.記憶細胞再次受到特異性抗原刺激后會被激活而成為分泌抗體的漿細胞(再次免疫應答);
5.記憶B細胞是長命細胞,可以存活數(shù)月甚至機體終生 。
★研盟生物★*抗體,Anti-Morphine抗體
Western Blotting 實驗步驟概述
1、 組織取材:組織塊稱重
2、 利用液氮、研缽粉碎組織塊
3、 加入RIPA緩沖液(每克組織3ml RIPA),PMSF(每克組織30μl,10 mg/ml PMSF),
利用Polytron進一步勻漿(15,000轉(zhuǎn)/分*1分鐘)維持4℃
4、 加入PMSF(每克組織30μl,10 mg/ml PMSF),冰上孵育30分鐘
5、 移入離心管4℃,約20,000g(約15,000轉(zhuǎn))15分鐘
6、 上清液為細胞裂解液可分裝-20℃保存
7、 進行Bradford比色法測定蛋白質(zhì)濃度
8、 取相同質(zhì)量的細胞裂解液(體積*蛋白質(zhì)濃度),并加等體積的2×電泳加樣緩沖液
9、 沸水浴中3分鐘
10、上樣
11、電泳(濃縮膠20mA,分離膠35mA)
12、電轉(zhuǎn)膜儀轉(zhuǎn)膜(100mA 40分鐘)
13、膜用麗春紅染色,膠用考馬斯亮藍染色
14、Westernblot 試劑盒顯色
15、分析比較記錄
簡單介紹:
產(chǎn)品別名:Morphine 6.
抗體來源:Rabbit or Mouse
保質(zhì)期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:Morphine is thought to produce reinforcement phenomena via stimulation of mu, delta, and kappa opioid receptors that regulate stress perception, pain control, reward behavior, and neurohormone secretion in reward-relevant brain systems. It has the highest affinity for mu, followed by delta and kappa. Rapid activation of the mu opioid receptor by morphine results in a euphoric phenotype, thus conferring the reinforcing effects of the drug. This activation is accompanied by extracellular dopamine release, which alters several events related to the cAMP signal transduction pathway. Of particular significance is that CREB seems to be modified by morphine, thereby affecting addictive behavioral phenomena, such as withdrawal symptoms.
新學期開學大減價*:
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Anti-Desmoglein 2 橋粒芯糖蛋白2抗體
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Anti-Nfasc186 神經(jīng)束蛋白186抗體
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