上海研盟生物有限公司*品質(zhì)供應(yīng)Anti-PPP2R1A抗體,我公司銷售國內(nèi)外科研產(chǎn)品,如生物試劑、生化試劑,一抗二抗,各種標記抗體,Elisa酶聯(lián)免疫試劑盒,放免試劑盒,培養(yǎng)基,各種動物血清等產(chǎn)品,歡迎廣大新老客戶前來咨詢訂購,訂購:或。
中文名稱:蛋白質(zhì)磷酸酶2調(diào)節(jié)亞基1A抗體
英文名稱:Anti-PPP2R1A antibody
保質(zhì)期: 1年
蛋白質(zhì)磷酸酶2調(diào)節(jié)亞基1A抗體,Anti-PPP2R1A抗體背景介紹:Protein phosphatase 2A (PP2A) is the most abundant phosphatase specific for serine and threonine phosphorylation in mammalian cells. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit alpha (PPP2R1A) serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory beta subunit.
相關(guān)標記抗體:HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體
蛋白質(zhì)磷酸酶2調(diào)節(jié)亞基1A抗體溶解方法:
方法1:我們的抗體(凍干粉)已經(jīng)包含了0.01M PBS、1% BSA和0.1% 防腐劑(疊氮鈉或*),您只需要加入滅菌的雙蒸水就行了??贵w溶解后,置于-20℃保存,分裝后使用,避免反復(fù)凍融,可保證至少1年有效;
方法2:也可以只加入一半體積的雙蒸水,再用一半體積的甘油補足,置于-20℃保存,這樣抗體不會凍,有利于抗體的穩(wěn)定。
后續(xù)試驗時,再使用對應(yīng)的緩沖液稀釋成工作液,即用即配。
我們的抗體(凍干粉),在常溫放置下,至少一個月有效;若在-20℃下保存(*),至少三年有效。
蛋白質(zhì)磷酸酶2調(diào)節(jié)亞基1A抗體,Anti-PPP2R1A抗體簡單介紹:
產(chǎn)品別名:PP2A; PP2A subunit A PR65-alpha isoform; PP2A subunit A R1 alpha isoform; PP2A, subunit A, PR65-alpha isoform; PP2A, subunit A, R1-alpha isoform; PPP2R1A; ppp2r1a-b; PR65; PR65A; Protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a ; Protein phosphatase 2 (formerly 2A), regulatory subunit A,; Protein phosphatase 2 regulatory subunit A alpha isoform; Protein phosphatase 2, 65-KD regulatory subunit A, alpha; Protein phosphatase 2, regulatory subunit A, alpha isoform; Protein phosphatase 2, structural/regulatory subunit A, alpha; PPP2R1A; Serine threonine protein phosphatase 65 kDa; Serine/threonine protein phosphatase 2A 65 KDA regulatory subunit A alpha isoform; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; Alpha isoform of regulatory subunit A, protein phosphatase; DKFZp470L0914; Medium tumor antigen-associated 61 kDa protein; 2AAA_HUMAN.
抗體來源:Rabbit or Mouse
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
免疫組化操作步驟:
(一)、儀器設(shè)備
1、 18cm不銹鋼高壓鍋或電爐或用微波爐
2、 水浴鍋
(二)、試 劑
1、 PBS緩沖液(pH7.2―7.4)
2、 0.01mol/L檸檬酸鈉緩沖液(CB,pH6.0,1000ml)
3、 3%甲醇―H2O2溶液:用30% H2O2 和80%甲醇溶液配制
4、 風裱劑:
a. 甘油和0.5mmol/L碳酸鹽緩沖液(pH9.0–9.5)等量混合
b. 油和TBS(PBS)配制
5、 TBS/PBS pH9.0–9.5,適用于熒光纖維鏡標本;pH7.0-7.4適合光學纖維標本
(三)、操作流程
1、 脫蠟和水化:脫蠟前應(yīng)將組織芯片在室溫中放置60分鐘或60℃恒溫箱中烘烤20分鐘
a. 組織芯片置于二甲苯中浸泡10分鐘,更換二甲苯后在浸泡10分鐘
b. 無水乙醇中浸泡五分鐘
c. 95%乙醇中浸泡五分鐘
d. 75%乙醇中浸泡五分鐘
2、 抗原修復(fù):用于福爾馬林固定的石蠟包埋組織芯片
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