上海研盟生物科技有限公司Anti-OLIG1抗體*,主要應(yīng)用于WB、IHC、IF、ELISA、流式細(xì)胞術(shù)等實(shí)驗(yàn)中。說(shuō)明書隨貨發(fā)送,您也可以直接我司在線客服索取??头?/span>
英文名稱:Anti-OLIG1 antibody
中文名稱:少突膠質(zhì)細(xì)胞轉(zhuǎn)錄因子1
少突膠質(zhì)細(xì)胞轉(zhuǎn)錄因子1保存條件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
少突膠質(zhì)細(xì)胞轉(zhuǎn)錄因子1,Anti-OLIG1抗體抗原修復(fù)方法:
方法1:沸水浴修復(fù),將盛有修復(fù)液和玻片的燒杯置于沸水浴環(huán)境,保持外部沸騰狀態(tài)15min,自然冷卻至室溫。
方法2:微波修復(fù),將盛有修復(fù)液和玻片的燒杯置于微波爐中,高火5min,?;?min,中火5min,自然冷卻至室溫。
方法3:高壓修復(fù),修復(fù)液加入高壓鍋加熱至沸騰,放入玻片,封蓋加壓持續(xù)加熱至噴氣時(shí)開始計(jì)時(shí)修復(fù)2min,自然冷卻至室溫。
方法4:使用*修復(fù),將*修復(fù)液加入到組織上,37℃,消化30min
★研盟生物★少突膠質(zhì)細(xì)胞轉(zhuǎn)錄因子1,Anti-OLIG1抗體
抗原修復(fù),必須注意以下問題:
1、修復(fù)液的選擇。
0.01M枸櫞酸(pH6.0):應(yīng)用zui多的抗原修復(fù)液,適用于大多數(shù)的抗原,用該液修復(fù)后的抗原表達(dá)增強(qiáng),抗原的定性和定位很理想,結(jié)果不錯(cuò);
0.05M EDTA(pH8.0):應(yīng)用較多的抗原修復(fù)液,修復(fù)能力較強(qiáng),對(duì)于保存時(shí)間較長(zhǎng)的切片或目的蛋白表達(dá)較弱的組織有很好的修復(fù)效果,需要控制好修復(fù)時(shí)間,否則容易出現(xiàn)較重的背景;
0.01M TBS(pH7.4):中性修復(fù)液,適用于大多數(shù)抗原,對(duì)于核定位蛋白有較好的修復(fù)效果;
*:應(yīng)用較多的抗原修復(fù)液,適用于大多數(shù)的抗原,使用溫度37℃,對(duì)于容易脫片的切片有很好的保護(hù)作用。
*:應(yīng)用較多的抗原修復(fù)液,適用于大多數(shù)的抗原
2.抗原熱修復(fù)時(shí)應(yīng)選擇的溫度。
據(jù)實(shí)驗(yàn)認(rèn)為溫度在92℃-98℃是合適的,尤其在95℃為,這是因?yàn)椋海?)這種溫度未達(dá)沸騰,切片不容易脫離載玻片;(2)能夠解離和破壞與蛋白交聯(lián)的甲醛醛鍵等,處理時(shí)可以隨意選擇和確定抗原修復(fù)的作用時(shí)間。如果選擇高壓修復(fù),因?yàn)闇囟容^高,時(shí)間不宜過(guò)長(zhǎng)
3.抗原修復(fù)時(shí)有效溫度所需持續(xù)時(shí)間根據(jù)各單位的設(shè)備條件以及使用的儀器不同,抗原修復(fù)時(shí)在有效的溫度范圍內(nèi)所持續(xù)的時(shí)間也不一樣。
4.抗原修復(fù)液必須遵循自然降溫規(guī)律,否則效果不好或達(dá)不到抗原修復(fù)的目的。
5.盡量使用足量的抗原修復(fù)液,防止切片干涸。
6.切片必須附貼牢固,否則發(fā)生掉片。
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簡(jiǎn)單介紹:
產(chǎn)品別名:Oligo 1; Oligo1; Oligodendrocyte lineage transcription factor 1; Oligodendrocyte specific bHLH transcription factor 1; Oligodendrocyte transcription factor 1. Basic domain helix loop helix protein class B 6; Basic domain helix loop helix protein class B6; BHLH B6; BHLHB 6; BHLHB6; bHLHe21; Class B basic helix-loop-helix protein 6; Class E basic helix-loop-helix protein 21; Olig 1; Olig1; OLIG1_HUMAN.
抗體來(lái)源:Rabbit or Mouse
保質(zhì)期:1年
克隆類型:Polyclonal or monoclonal
性 狀:Lyophilized or Liquid
濃 度:1mg/1ml
背景介紹:The oligodendrocyte lineage-specific basic helix-loop-helix (OLIG) family of transcription factors include OLIG1-OLIG3, which differ in tissue expression. OLIG1 and OLIG2 are specifically expressed in nervous tissue as gene regulators of oligodendrogenesis. OLIG2 is more widely expressed in embryonic brain than OLIG1, while OLIG3 is primarily expressed in non-neural tissues. OLIG1 and OLIG2 interact with the Nkx-2.2 homeodomain protein, which is responsible for directing ventral neuronal patterning in response to graded Sonic hedgehog signaling in the embryonic neural tube. These interactions between OLIG proteins and Nkx-2.2 appear to promote the formation of alternate cell types by inhibiting V3 interneuron development. OLIG1 and OLIG2 are abundantly expressed in oligodendroglioma and nearly absent in astrocytomas. Therefore, OLIG proteins are candidates for molecular markers of human glial brain tumors, which are the most common primary malignancies of the human brain.
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