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Anti Active Gα13 Mouse Monoclonal Antibody

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參   考   價(jià): 4800

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ActiveGα13GTP[26902][30µL]CatalogNumber:26902GeneSymbol:Gna13Description:AntiActiveGα13MouseMonoclonalAntibodyBackground:HeterotrimericGproteinsareessentialcellularsignaltransducers

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Active Gα13 GTP [26902]
[30 µL]


Catalog Number:  26902
Gene Symbol:  Gna13
Description:   Anti Active Gα13 Mouse Monoclonal Antibody
Background:   Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored.
Immunogen:   Recombinant full length active Gα13 protein
Applications:   IP, IHC
Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                   1:100 dilution for IHC
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG2b
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
Download Datasheet:  
Immunoprecipitation/Western blot:  
IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti active Gα13 GTP mouse monoclonal antibody (Cat. # 26902), these proteins were separated by SDS/PAGE and western blotted with anti Gα13 mouse monoclonal antibody (Cat. # 26004).
Immunohistochemistry:  
Staining of MEF cells (treated with PDGF) with anti Ga13 GTP monoclonal antibody showing the localization of Gα13 GTP in the leading edge of migrating cells.



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Publications:
1.  
    J Biol Chem. 2011 Sep 2;286(35):31055-61
2.  
    Br J Pharmacol. 2011 Feb;162(3):733-48
3.  
    Carcinogenesis (2010)
4.  
    J Biol Chem. 2009 Oct 2;284(40):27409-15





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