人結(jié)腸癌細(xì)胞HCT116

人結(jié)腸癌細(xì)胞HCT116 是1979年BrattainM等分離建立的3株惡性細(xì)胞之一；產(chǎn)生CEA、角蛋白。在半固體瓊脂糖培養(yǎng)基中形成克隆。HCT116在無胸腺的裸鼠有致瘤性，形成上皮樣的腫瘤。

細(xì)胞名稱：人結(jié)腸癌細(xì)胞

細(xì)胞簡稱：HCT116細(xì)胞

細(xì)胞別稱：HCT-116; HCT.116; HCT_116; HCT116; CoCL2

產(chǎn)品貨號：TCH-C185

種屬來源：人

組織來源：結(jié)腸

特征：結(jié)腸癌

細(xì)胞形態(tài)：上皮細(xì)胞樣

生長特性：貼壁生長

培養(yǎng)體系： McCoy's 5A+10%FBS+1%P/S

配套培養(yǎng)基貨號：TCH-G185

傳代比例：1:3-1:8，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲存

質(zhì)量檢測：細(xì)菌、真菌、支原體檢測均為陰性

實驗耗材：細(xì)胞培養(yǎng)瓶，錐形搖瓶,細(xì)胞培養(yǎng)皿

實驗室儀器：CO2培養(yǎng)箱

人結(jié)腸癌細(xì)胞HCT116 使用說明書

參考文獻(xiàn)：

"Park SM, et al. The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2. Genes Dev 22(7): 894-907, 2008. PubMed:

Schroy PC, et al. Detection of p21ras mutations in colorectal adenomas and carcinomas by enzyme-linked immunosorbent assay. Cancer 76: 201-209, 1995. PubMed: 8625092

Brattain MG, et al. Heterogeneity of malignant cells from a human colonic carcinoma. Cancer Res. 41: 1751-1756, 1981. PubMed: 7214343

Sun L, et al. Autocrine transforming growth factor-beta 1 and beta 2 expression is increased by cell crowding and quiescence in colon carcinoma cells. Exp. Cell Res. 214: 215-224, 1994. PubMed: 8082724

Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479

Brattain MG, et al. Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. J. Natl. Cancer Inst. 69: 767-771, 1982. PubMed: 6956756

Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064

Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

Kutchera W, et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486

Wang R, et al. Cellular adherence elicits ligand-independent activation of the Met cell-surface receptor. Proc. Natl. Acad. Sci. USA 93: 8425-8430, 1996. PubMed: 8710887"