人單核細(xì)胞巨噬細(xì)胞分化關(guān)聯(lián)蛋白檢測試劑盒定量檢測,具有靈敏性高,特異性強(qiáng),重復(fù)性好,性價(jià)比高,線性好;操作簡單,精確度高;售前售后完善,隨時(shí)來實(shí)驗(yàn)室現(xiàn)場觀看,常見ELISA試劑盒系列:人,大鼠,小鼠:TNF-α、IL-1β、IL-7、IL-32、IL-21、IL-27、IL-23、IFN-γ、TGF-α、TGF-β1等。
英文簡稱:Human Monocyte To Macrophage Differentiation Associated Protein (MMA) ELISA Kit
人單核細(xì)胞巨噬細(xì)胞分化關(guān)聯(lián)蛋白檢測試劑盒注意事項(xiàng)以下四點(diǎn):
1,封閉:以下包以后是無關(guān)的蛋白質(zhì)溶液濃度高,涂層技術(shù)。隨函附上使許多不相關(guān)的蛋白質(zhì)充填這些空隙,和攪擾物質(zhì)的免疫排擠和吸附進(jìn)程。ELISA試劑盒常用的密封:0。05% - 0。5%牛血清白蛋白;10%或1%明膠牛血清;脫脂奶粉,報(bào)價(jià)相對(duì)低廉,可用于高濃度(5%~10%);和一些稀有的運(yùn)用各種動(dòng)物血清(首要是為了消除類似的蛋白和酪蛋白等攪擾)。但究竟挑選啥,依據(jù)試驗(yàn)的詳細(xì)實(shí)習(xí)。
2,洗板:能夠說,在中操作,清洗是在首要的關(guān)鍵技術(shù)。因?yàn)榫郾揭蚁┑人芰蠈?duì)蛋白質(zhì)的吸附是普遍存在的,為了完成自在酶與客觀相的符號(hào)的別離,在板料孔中殘留游離和吸附的非特異性攪擾物質(zhì)的去掉,洗刷,并應(yīng)將攪擾物質(zhì)洗刷下來的非特異性吸附。所以在洗衣板會(huì)有必定的誤差,非常人的要素(當(dāng)然也有洗衣機(jī)除條件),是不完整的或弦清孔,體系的影響是如此靈敏,但不小。
3,加抗體(抗標(biāo)本和兩個(gè)):留意的槍頭,槍頭。樣品用稀釋稀釋,也能夠用稀釋的閉解。假如要增加兩個(gè)電阻,但也要留意兩個(gè)反作業(yè)濃度的廢物,太高,太低的光的色彩。
4,色彩:色彩體系有許多,咱們開端做,挑選恰當(dāng)?shù)纳鼠w系。ELISA試劑盒留意色彩體系酶底物物加硫柳泵節(jié)省,組合物加*。7,每次都盡可能的陰和陽空三操控,如剖析疑問的呈現(xiàn)。YB-TGFb2-Rb 兔轉(zhuǎn)化生長因子β2(TGFβ2) ELISA Kit Rabbit Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit Oryctolagus cuniculus Rabbit 15.625-1000pg/mL 5.2pg/mL sandwich 96T/48T 825/577.5 12 months
YB-TGFb2-Si 猴轉(zhuǎn)化生長因子β2(TGFβ2) ELISA Kit Monkey Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit Rhesus monkey Simian 31.25-2000pg/mL 14.7pg/mL sandwich 96T/48T 982.5/687.8 12 months
YB-TGFb3-c 犬轉(zhuǎn)化生長因子β3(TGFβ3) ELISA Kit Canine Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit Canis familiaris; Canine Dog 78.125-5000pg/mL 19.8pg/mL sandwich 96T/48T 920/644 12 months
YB-TGFb3-Ch 雞轉(zhuǎn)化生長因子β3(TGFβ3) ELISA Kit Chicken Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit Chicken Gallus 31.25-2000pg/mL 7.9pg/mL sandwich 96T/48T 872.6/610.8 12 months
YB-TGFb3-Eq 馬轉(zhuǎn)化生長因子β3(TGFβ3) ELISA Kit Equine Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit Equus caballus; Equine Horse 125-8000pg/mL 31pg/mL sandwich 96T/48T 991.2/693.9 12 months
YB-TGFb3-g 山羊轉(zhuǎn)化生長因子β3(TGFβ3) ELISA Kit Goat Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit Capra hircus; Caprine Goat 93.75-6000pg/mL 23.4pg/mL sandwich 96T/48T 991.2/693.9 12 months
YB-TGFb3-Gu 豚鼠轉(zhuǎn)化生長因子β3(TGFβ3) ELISA Kit Guinea pig Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit Cavia Guinea pig 31.25-2000pg/mL 7.6pg/mL sandwich 96T/48T 920/644 12 months
YB-TGFb3-Hu 人轉(zhuǎn)化生長因子β3(TGFβ3) ELISA Kit Human Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit Homo sapiens human 78.125-5000pg/mL 3.4pg/mL sandwich 96T/48T 801.3/560.9 12 months
YB-TGFb3-Mu 小鼠轉(zhuǎn)化生長因子β3(TGFβ3) ELISA Kit Mouse Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit Mus musculus mouse 15.625-1000pg/mL 6.3pg/mL sandwich 96T/48T 825/577.5 12 months
YB-TGFb3-p 豬轉(zhuǎn)化生長因子β3(TGFβ3) ELISA Kit Porcine Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit Sus scrofa; Porcine Pig 15.625-1000pg/mL 5.6pg/mL sandwich 96T/48T 967.5/677.3 12 months
YB-TGFb3-Ra 大鼠轉(zhuǎn)化生長因子β3(TGFβ3) ELISA Kit Rat Transforming Growth Factor Beta 3 (TGFb3) ELISA Kit Rattus norvegicus Rat 15.625-1000pg/mL 5.8pg/mL sandwich 96T/48T 872.6/610.8 12 months
YB-TGFbI-b 牛轉(zhuǎn)化生長因子β誘導(dǎo)蛋白(TGFβI) ELISA Kit Bovine Transforming Growth Factor Beta Induced Protein (TGFbI) ELISA Kit Bos taurus; Bovine Cattle 1.56-100ng/mL 0.39ng/mL sandwich 96T/48T 915/640.5 12 months
YB-TGFbI-Fi 魚轉(zhuǎn)化生長因子β誘導(dǎo)蛋白(TGFβI) ELISA Kit Fish Transforming Growth Factor Beta Induced Protein (TGFbI) ELISA Kit Danio rerio Zebrafish 7.812-500pg/mL 3.8pg/mL sandwich 96T/48T 1095/766.5 12 months
YB-TGFbI-Hu 人轉(zhuǎn)化生長因子β誘導(dǎo)蛋白(TGFβI) ELISA Kit