ELISA的方法檢測多肽與小鼠磷脂酶A2(PL-A2)測定試劑盒的結(jié)合情況。準備將合成多肽(其長度大約為8~10個氨基酸)包被于ELISA板,再加入小鼠磷脂酶A2(PL-A2)測定試劑盒,一抗、酶標二抗。請問在包被的過程中應(yīng)采取哪種包被緩沖液?固相載體是否要特殊處理?封閉液用BSA可以嗎?
1、多肽抗原的包被一般需先使其與無關(guān)蛋白質(zhì)如牛血清白蛋白質(zhì)(BSA)等偶聯(lián),借助于偶聯(lián)物與固相載體的吸附,間接地結(jié)合到固相載體表面。
2、小鼠磷脂酶A2(PL-A2)測定試劑盒包被用抗原或抗體的濃度,包被的溫度和時間,包被液的pH等應(yīng)根據(jù)試驗的特點和材料的性質(zhì)而選定。抗體和蛋白質(zhì)抗原一般采用pH9.6的碳酸鹽緩沖液作為稀釋液,也有用pH7.2的磷酸鹽緩沖液及pH7~8的Tris-HCL緩沖液作為稀釋液的。通常在ELISA板孔中加入包被液后,在4-8℃冰箱中放置過夜,37℃中保溫2小時被認為具有同等的包被效果。
3、zui常用的封閉劑是0.05%-0.5%的牛血清白蛋白,小鼠磷脂酶A2(PL-A2)測定試劑盒也有用10%的小牛血清或1%明膠作為封閉劑的。脫脂奶粉也是一種良好的封閉劑,其zui大的特點是價廉,可以高濃度使用(5%)。
顯色終止后出現(xiàn)黑色絮狀沉淀可能為包被板上的蛋白,酶標記物,檢測物,封閉液中蛋白變性以及顯色反應(yīng)TMB過量,不溶,沉淀出來。
小鼠磷脂酶A2(PL-A2)測定試劑盒其它優(yōu)勢產(chǎn)品如下:
klA527Mu 小鼠氧化低密度脂蛋白(OxLDL)ELISA試劑盒 ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL) Assay range:30 ng/L -1325 ng/L
klB908Mu 小鼠聚集蛋白聚糖(AGC)ELISA試劑盒 ELISA Kit for Aggrecan (AGC) Assay range:30 ng/L -1326 ng/L
klA411Mu 小鼠鈣非依賴性磷脂酶A2(iPLA2)ELISA試劑盒 ELISA Kit for Phospholipase A2, Calcium Independent (iPLA2) Assay range:30 ng/L -1327 ng/L
klA842Mu 小鼠脂蛋白a(Lpa)ELISA試劑盒 ELISA Kit for Lipoprotein, a (Lpa) Assay range:30 ng/L -1328 ng/L
klA204Mu 小鼠生長停滯特異性蛋白6(GAS6)ELISA試劑盒 ELISA Kit for Growth Arrest Specific Protein 6 (GAS6) Assay range:30 ng/L -1329 ng/L
klB706Mu 小鼠脂質(zhì)運載蛋白1(LCN1)ELISA試劑盒 ELISA Kit for Lipocalin 1 (LCN1) Assay range:30 ng/L -1330 ng/L
klA622Mu 小鼠*S轉(zhuǎn)移酶θ1(GSTθ1)ELISA試劑盒 ELISA Kit for Glutathione S Transferase Theta 1 (GSTt1) Assay range:30 ng/L -1331 ng/L
klB983Mu 小鼠微管關(guān)聯(lián)蛋白τ(MAPτ)ELISA試劑盒 ELISA Kit for Microtubule Associated Protein Tau (MAPt) Assay range:30 ng/L -1332 ng/L
klB995Mu 小鼠血幼素(HJV)ELISA試劑盒 ELISA Kit for Hemojuvelin (HJV) Assay range:30 ng/L -1333 ng/L
klA681Mu 小鼠乙醇脫氫酶1(ADH1)ELISA試劑盒 ELISA Kit for Alcohol Dehydrogenase 1 (ADH1) Assay range:30 ng/L -1334 ng/L
klC024Mu 小鼠線粒體乙醛脫氫酶(ALDM)ELISA試劑盒 ELISA Kit for Aldehyde Dehydrogenase, Mitochondrial (ALDM) Assay range:30 ng/L -1335 ng/L
klE866Mu 小鼠乙醇脫氫酶7(ADH7)ELISA試劑盒 ELISA Kit for Alcohol Dehydrogenase 7 (ADH7) Assay range:30 ng/L -1336 ng/L
klB329Mu 小鼠微管關(guān)聯(lián)蛋白2(MAP2)ELISA試劑盒 ELISA Kit for Microtubule Associated Protein 2 (MAP2) Assay range:30 ng/L -1337 ng/L
klA645Mu 小鼠晚期糖基化終末產(chǎn)物受體(AGER)ELISA試劑盒 ELISA Kit for Advanced Glycosylation End Product Specific Receptor (AGER) Assay range:30 ng/L -1338 ng/L
klA694Mu 小鼠含血管性血友病因子A域蛋白3A(vWA3A)ELISA試劑盒 ELISA Kit for Von Willebrand Factor A Domain Containing Protein 3A (vWA3A) Assay range:30 ng/L -1339 ng/L
klA139Mu 小鼠腫瘤壞死因子相關(guān)凋亡誘導(dǎo)配體(TRAIL)ELISA試劑盒 ELISA Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) Assay range:30 ng/L -1340 ng/L
klA919Mu 小鼠二氫*脫氫酶(DLD)ELISA試劑盒 ELISA Kit for Dihydrolipoyl Dehydrogenase (DLD) Assay range:30 ng/L -1341 ng/L
klB539Mu 小鼠血清淀粉樣P物質(zhì)(SAP)ELISA試劑盒 ELISA Kit for Serum Amyloid P Component (SAP) Assay range:30 ng/L -1342 ng/L
klB265Mu 小鼠胰多肽(PP)ELISA試劑盒 ELISA Kit for Pancreatic Polypeptide (PP) Assay range:30 ng/L -1343 ng/L
klB311Mu 小鼠阿黑皮素原(POMC)ELISA試劑盒 ELISA Kit for Proopiomelanocortin (POMC) Assay range:30 ng/L -1344 ng/L
klB686Mu 小鼠B-細胞激活因子(BAFF)ELISA試劑盒 ELISA Kit for B-Cell Activating Factor (BAFF) Assay range:30 ng/L -1345 ng/L
klA442Mu 小鼠阻抑素(PHB)ELISA試劑盒 ELISA Kit for Prohibitin (PHB) Assay range:30 ng/L -1346 ng/L
klB685Mu 小鼠整合素αM(ITGαM)ELISA試劑盒 ELISA Kit for Integrin Alpha M (ITGaM) Assay range:30 ng/L -1347 ng/L
klB966Mu 小鼠多配體蛋白聚糖1(SDC1)ELISA試劑盒 ELISA Kit for Syndecan 1 (SDC1) Assay range:30 ng/L -1348 ng/L
klA977Mu 小鼠盤狀結(jié)構(gòu)域受體家族成員1(DDR1)ELISA試劑盒 ELISA Kit for Discoidin Domain Receptor Family, Member 1 (DDR1) Assay range:30 ng/L -1349 ng/L
klB317Mu 小鼠穿孔素1(PRF1)ELISA試劑盒 ELISA Kit for Perforin 1 (PRF1) Assay range:30 ng/L -1350 ng/L
klB017Mu 小鼠α2-巨球蛋白(α2M)ELISA試劑盒 ELISA Kit for Alpha-2-Macroglobulin (a2M) Assay range:30 ng/L -1351 ng/L
klB063Mu 小鼠泛素結(jié)合酶E2A(UBE2A)ELISA試劑盒 ELISA Kit for Ubiquitin Conjugating Enzyme E2A (UBE2A) Assay range:30 ng/L -1352 ng/L
klG208Mu 小鼠叉頭框蛋白A2(FOXA2)ELISA試劑盒 ELISA Kit for Forkhead Box Protein A2 (FOXA2) Assay range:30 ng/L -1353 ng/L