ELISA的方法檢測多肽與小鼠水通道蛋白4(AQP-4)測定試劑盒的結(jié)合情況。準(zhǔn)備將合成多肽(其長度大約為8~10個氨基酸)包被于ELISA板,再加入小鼠水通道蛋白4(AQP-4)測定試劑盒,一抗、酶標(biāo)二抗。請問在包被的過程中應(yīng)采取哪種包被緩沖液?固相載體是否要特殊處理?封閉液用BSA可以嗎?
1、多肽抗原的包被一般需先使其與無關(guān)蛋白質(zhì)如牛血清白蛋白質(zhì)(BSA)等偶聯(lián),借助于偶聯(lián)物與固相載體的吸附,間接地結(jié)合到固相載體表面。
2、小鼠水通道蛋白4(AQP-4)測定試劑盒包被用抗原或抗體的濃度,包被的溫度和時間,包被液的pH等應(yīng)根據(jù)試驗的特點和材料的性質(zhì)而選定??贵w和蛋白質(zhì)抗原一般采用pH9.6的碳酸鹽緩沖液作為稀釋液,也有用pH7.2的磷酸鹽緩沖液及pH7~8的Tris-HCL緩沖液作為稀釋液的。通常在ELISA板孔中加入包被液后,在4-8℃冰箱中放置過夜,37℃中保溫2小時被認(rèn)為具有同等的包被效果。
3、zui常用的封閉劑是0.05%-0.5%的牛血清白蛋白,小鼠水通道蛋白4(AQP-4)測定試劑盒也有用10%的小牛血清或1%明膠作為封閉劑的。脫脂奶粉也是一種良好的封閉劑,其zui大的特點是價廉,可以高濃度使用(5%)。
顯色終止后出現(xiàn)黑色絮狀沉淀可能為包被板上的蛋白,酶標(biāo)記物,檢測物,封閉液中蛋白變性以及顯色反應(yīng)TMB過量,不溶,沉淀出來。
小鼠水通道蛋白4(AQP-4)測定試劑盒其它優(yōu)勢產(chǎn)品如下:
klD076Mu 小鼠碳酸酐酶Ⅸ(CA9)ELISA試劑盒 ELISA Kit for Carbonic Anhydrase IX (CA9) Assay range:30 ng/L -1414 ng/L
klF792Mu 小鼠味覺受體1型成員2(TAS1R2)ELISA試劑盒 ELISA Kit for Taste Receptor Type 1 Member 2 (TAS1R2) Assay range:30 ng/L -1415 ng/L
klA677Mu 小鼠*Ⅻ(F12)ELISA試劑盒 ELISA Kit for Coagulation Factor XII (F12) Assay range:30 ng/L -1416 ng/L
klB582Mu 小鼠IgE-Fc片段受體Ⅱ(FcεRⅡ)ELISA試劑盒 ELISA Kit for Receptor II For The Fc Region Of Immunoglobulin E (FceRII) Assay range:30 ng/L -1417 ng/L
klC503Mu 小鼠G蛋白β1(GNβ1)ELISA試劑盒 ELISA Kit for G Protein Beta 1 (GNb1) Assay range:30 ng/L -1418 ng/L
klB694Mu 小鼠白介素25(IL25)ELISA試劑盒 ELISA Kit for Interleukin 25 (IL25) Assay range:30 ng/L -1419 ng/L
klL154Mu 小鼠成纖維細(xì)胞生長因子15(FGF15)ELISA試劑盒 ELISA Kit for Fibroblast Growth Factor 15 (FGF15) Assay range:30 ng/L -1420 ng/L
klF557Mu 小鼠線粒體解偶聯(lián)蛋白1(UCP1)ELISA試劑盒 ELISA Kit for Uncoupling Protein 1, Mitochondrial (UCP1) Assay range:30 ng/L -1421 ng/L
klK166Mu 小鼠*豐富重復(fù)LGI家族成員3(LGI3)ELISA試劑盒 ELISA Kit for Leucine Rich Repeat LGI Family, Member 3 (LGI3) Assay range:30 ng/L -1422 ng/L
klB312Mu 小鼠分泌性白細(xì)胞蛋白酶抑制因子(SLPI)ELISA試劑盒 ELISA Kit for Secretory Leukocyte Peptidase Inhibitor (SLPI) Assay range:30 ng/L -1423 ng/L
klF880Mu 小鼠分泌型卷曲相關(guān)蛋白1(SFRP1)ELISA試劑盒 ELISA Kit for Secreted Frizzled Related Protein 1 (SFRP1) Assay range:30 ng/L -1424 ng/L
klB699Mu 小鼠血管生成素樣蛋白3(ANGPTL3)ELISA試劑盒 ELISA Kit for Angiopoietin Like Protein 3 (ANGPTL3) Assay range:30 ng/L -1425 ng/L
klD466Mu 小鼠低氧誘導(dǎo)因子2α(HIF2α)ELISA試劑盒 ELISA Kit for Hypoxia Inducible Factor 2 Alpha (HIF2a) Assay range:30 ng/L -1426 ng/L
klB523Mu 小鼠趨化因子C-X3-C-基元受體1(CX3CR1)ELISA試劑盒 ELISA Kit for Chemokine C-X3-C-Motif Receptor 1 (CX3CR1) Assay range:30 ng/L -1427 ng/L
klL624Mu 小鼠107kDa肌醇多聚磷酸-4-磷酸酶Ⅰ型(INPP4A)ELISA試劑盒 ELISA Kit for Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) Assay range:30 ng/L -1428 ng/L
klC260Mu 小鼠融合蛋白(FUS)ELISA試劑盒 ELISA Kit for Fusion (FUS) Assay range:30 ng/L -1429 ng/L
klC702Mu 小鼠視神經(jīng)病變誘導(dǎo)反應(yīng)蛋白(OPTN)ELISA試劑盒 ELISA Kit for Optineurin (OPTN) Assay range:30 ng/L -1430 ng/L
klE095Mu 小鼠原鈣黏素15(PCDH15)ELISA試劑盒 ELISA Kit for Protocadherin 15 (PCDH15) Assay range:30 ng/L -1431 ng/L
klB847Mu 小鼠極低密度脂蛋白(VLDL)ELISA試劑盒 ELISA Kit for Very Low Density Lipoprotein (VLDL) Assay range:30 ng/L -1432 ng/L
klC563Mu 小鼠白細(xì)胞衍生趨化因子1(LECT1)ELISA試劑盒 ELISA Kit for Leukocyte Cell Derived Chemotaxin 1 (LECT1) Assay range:30 ng/L -1433 ng/L
klA768Mu 小鼠足盂蛋白(PCX)ELISA試劑盒 ELISA Kit for Podocalyxin (PCX) Assay range:30 ng/L -1434 ng/L
klC387Mu 小鼠夏科雷登氏結(jié)晶蛋白(CLC)ELISA試劑盒 ELISA Kit for Charcot Leyden Crystal Protein (CLC) Assay range:30 ng/L -1435 ng/L
klJ246Mu 小鼠DNA甲基轉(zhuǎn)移酶3B(DNMT3B)ELISA試劑盒 ELISA Kit for DNA Methyltransferase 3B (DNMT3B) Assay range:30 ng/L -1436 ng/L
klD475Mu 小鼠胰高血糖素樣肽1受體(GLP1R)ELISA試劑盒 ELISA Kit for Glucagon Like Peptide 1 Receptor (GLP1R) Assay range:30 ng/L -1437 ng/L
klB201Mu 小鼠接觸蛋白1(CNTN1)ELISA試劑盒 ELISA Kit for Contactin 1 (CNTN1) Assay range:30 ng/L -1438 ng/L
klB918Mu 小鼠蛋白激酶Cα(PKCα)ELISA試劑盒 ELISA Kit for Protein Kinase C Alpha (PKCa) Assay range:30 ng/L -1439 ng/L
klG316Mu 小鼠Corin蛋白(CRN)ELISA試劑盒 ELISA Kit for Corin (CRN) Assay range:30 ng/L -1440 ng/L
klB364Mu 小鼠乳腺癌易感蛋白2(BRCA2)ELISA試劑盒 ELISA Kit for Breast Cancer Susceptibility Protein 2 (BRCA2) Assay range:30 ng/L -1441 ng/L