ELISA的方法檢測(cè)多肽與小鼠水通道蛋白3(AQP-3)測(cè)定試劑盒的結(jié)合情況。準(zhǔn)備將合成多肽(其長(zhǎng)度大約為8~10個(gè)氨基酸)包被于ELISA板,再加入小鼠水通道蛋白3(AQP-3)測(cè)定試劑盒,一抗、酶標(biāo)二抗。請(qǐng)問(wèn)在包被的過(guò)程中應(yīng)采取哪種包被緩沖液?固相載體是否要特殊處理?封閉液用BSA可以嗎?
1、多肽抗原的包被一般需先使其與無(wú)關(guān)蛋白質(zhì)如牛血清白蛋白質(zhì)(BSA)等偶聯(lián),借助于偶聯(lián)物與固相載體的吸附,間接地結(jié)合到固相載體表面。
2、小鼠水通道蛋白3(AQP-3)測(cè)定試劑盒包被用抗原或抗體的濃度,包被的溫度和時(shí)間,包被液的pH等應(yīng)根據(jù)試驗(yàn)的特點(diǎn)和材料的性質(zhì)而選定。抗體和蛋白質(zhì)抗原一般采用pH9.6的碳酸鹽緩沖液作為稀釋液,也有用pH7.2的磷酸鹽緩沖液及pH7~8的Tris-HCL緩沖液作為稀釋液的。通常在ELISA板孔中加入包被液后,在4-8℃冰箱中放置過(guò)夜,37℃中保溫2小時(shí)被認(rèn)為具有同等的包被效果。
3、zui常用的封閉劑是0.05%-0.5%的牛血清白蛋白,小鼠水通道蛋白3(AQP-3)測(cè)定試劑盒也有用10%的小牛血清或1%明膠作為封閉劑的。脫脂奶粉也是一種良好的封閉劑,其zui大的特點(diǎn)是價(jià)廉,可以高濃度使用(5%)。
顯色終止后出現(xiàn)黑色絮狀沉淀可能為包被板上的蛋白,酶標(biāo)記物,檢測(cè)物,封閉液中蛋白變性以及顯色反應(yīng)TMB過(guò)量,不溶,沉淀出來(lái)。
小鼠水通道蛋白3(AQP-3)測(cè)定試劑盒其它優(yōu)勢(shì)產(chǎn)品如下:
klB650Mu 小鼠主要堿性蛋白(MBP)ELISA試劑盒 ELISA Kit for Major Basic Protein (MBP) Assay range:30 ng/L -1442 ng/L
klL300Mu 小鼠CD5抗原樣蛋白(CD5L)ELISA試劑盒 ELISA Kit for CD5 Antigen Like Protein (CD5L) Assay range:30 ng/L -1443 ng/L
klC106Mu 小鼠骨成型蛋白10(BMP10)ELISA試劑盒 ELISA Kit for Bone Morphogenetic Protein 10 (BMP10) Assay range:30 ng/L -1444 ng/L
klC937Mu 小鼠鞘氨醇1磷酸酯受體1(S1PR1)ELISA試劑盒 ELISA Kit for Sphingosine 1 Phosphate Receptor 1 (S1PR1) Assay range:30 ng/L -1445 ng/L
klB792Mu 小鼠S100鈣結(jié)合蛋白A8(S100A8)ELISA試劑盒 ELISA Kit for S100 Calcium Binding Protein A8 (S100A8) Assay range:30 ng/L -1446 ng/L
klB793Mu 小鼠S100鈣結(jié)合蛋白A9(S100A9)ELISA試劑盒 ELISA Kit for S100 Calcium Binding Protein A9 (S100A9) Assay range:30 ng/L -1447 ng/L
klJ822Mu 小鼠B-細(xì)胞κ-輕肽基因增強(qiáng)子抑制因子激酶β(IκBKβ)ELISA試劑盒 ELISA Kit for Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb) Assay range:30 ng/L -1448 ng/L
klA549Mu 小鼠角蛋白9(KRT9)ELISA試劑盒 ELISA Kit for Keratin 9 (KRT9) Assay range:30 ng/L -1449 ng/L
klB817Mu 小鼠多功能蛋白聚糖(VS)ELISA試劑盒 ELISA Kit for Versican (VS) Assay range:30 ng/L -1450 ng/L
klC228Mu 小鼠密封蛋白(OCLN)ELISA試劑盒 ELISA Kit for Occludin (OCLN) Assay range:30 ng/L -1451 ng/L
klF292Mu 小鼠封閉蛋白2(CLDN2)ELISA試劑盒 ELISA Kit for Claudin 2 (CLDN2) Assay range:30 ng/L -1452 ng/L
klD089Mu 小鼠溶酶體酸性磷酸酶2(ACP2)ELISA試劑盒 ELISA Kit for Acid Phosphatase 2, Lysosomal (ACP2) Assay range:30 ng/L -1453 ng/L
klB280Mu 小鼠組織蛋白酶D(CTSD)ELISA試劑盒 ELISA Kit for Cathepsin D (CTSD) Assay range:30 ng/L -1454 ng/L
klB120Mu 小鼠*酶(Arg)ELISA試劑盒 ELISA Kit for Arginase (Arg) Assay range:30 ng/L -1455 ng/L
klB498Mu 小鼠蛋白二硫化物異構(gòu)酶A2(PDIA2)ELISA試劑盒 ELISA Kit for Protein Disulfide Isomerase A2 (PDIA2) Assay range:30 ng/L -1456 ng/L
klM546Mu 小鼠Lin-28同源物A(LIN28A)ELISA試劑盒 ELISA Kit for Lin-28 Homolog A (LIN28A) Assay range:30 ng/L -1457 ng/L
klB884Mu 小鼠受磷蛋白(PLN)ELISA試劑盒 ELISA Kit for Phospholamban (PLN) Assay range:30 ng/L -1458 ng/L
klE676Mu 小鼠再生胰島衍生蛋白3γ(REG3γ)ELISA試劑盒 ELISA Kit for Regenerating Islet Derived Protein 3 Gamma (REG3g) Assay range:30 ng/L -1459 ng/L
klC773Mu 小鼠蘭尼定受體1(RYR1)ELISA試劑盒 ELISA Kit for Ryanodine Receptor 1, Skeletal (RYR1) Assay range:30 ng/L -1460 ng/L
klF663Mu 小鼠核因子I/B(NFIB)ELISA試劑盒 ELISA Kit for Nuclear Factor I/B (NFIB) Assay range:30 ng/L -1461 ng/L
klB564Mu 小鼠干擾素調(diào)節(jié)因子1(IRF1)ELISA試劑盒 ELISA Kit for Interferon Regulatory Factor 1 (IRF1) Assay range:30 ng/L -1462 ng/L
klH522Mu 小鼠腎連蛋白(NPNT)ELISA試劑盒 ELISA Kit for Nephronectin (NPNT) Assay range:30 ng/L -1463 ng/L
klB483Mu 小鼠胰彈性蛋白酶(ELA1)ELISA試劑盒 ELISA Kit for Elastase 1, Pancreatic (ELA1) Assay range:30 ng/L -1464 ng/L
klC010Mu 小鼠蛋白Z依賴性蛋白酶抑制因子(ZPI)ELISA試劑盒 ELISA Kit for Protein Z Dependent Protease Inhibitor (ZPI) Assay range:30 ng/L -1465 ng/L
klD696Mu 小鼠安定結(jié)合抑制因子(DBI)ELISA試劑盒 ELISA Kit for Diazepam Binding Inhibitor (DBI) Assay range:30 ng/L -1466 ng/L
klG515Mu 小鼠肥大細(xì)胞類*1(CMA1)ELISA試劑盒 ELISA Kit for Chymase 1, Mast Cell (CMA1) Assay range:30 ng/L -1467 ng/L
klB034Mu 小鼠粘蛋白4(MUC4)ELISA試劑盒 ELISA Kit for Mucin 4 (MUC4) Assay range:30 ng/L -1468 ng/L
klB226Mu 小鼠*結(jié)合球蛋白(CBG)ELISA試劑盒 ELISA Kit for Corticosteroid Binding Globulin (CBG) Assay range:30 ng/L -1469 ng/L
klB705Mu 小鼠防御素α1(DEFα1)ELISA試劑盒 ELISA Kit for Defensin Alpha 1, Neutrophil (DEFa1) Assay range:30 ng/L -1470 ng/L
klB345Mu 小鼠四旋蛋白30(TSPAN30)ELISA試劑盒 ELISA Kit for Tetraspanin 30 (TSPAN30) Assay range:30 ng/L -1471 ng/L
klA363Mu 小鼠血小板內(nèi)皮細(xì)胞粘附分子1(PECAM1)ELISA試劑盒 ELISA Kit for Plaet/Endothelial Cell Adhesion Molecule (PECAM1) Assay range:30 ng/L -1472 ng/L